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Then, the target proteins or subdomains of them containing the goal lysine may be produced recombinantly and the methylation also tested at protein degree. The horizontal axis represents the H3 sequence and the vertical axis represents the amino acids by which the corresponding row is mutated. A) Discrimination elements of NSD1 for the recognition of amino acid residues at the positions next to the target lysine (K36 within the experiment proven here).
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